Analyzing correlations between GNSS retrieved precipitable water vapor and land surface temperature after earthquakes occurrence.

Earthquake is a common and destructive natural disaster. The enormous amount of energy released from seismic events can result in anomalous land surface temperature (LST) and catalyze the accumulation of water vapor in the atmosphere. The majority of previous works are not consensual concerning precipitable water vapor (PWV) and LST after the earthquake. Here, we utilized multi-source data to analyze the changes of PWV and LST anomaly after three Ms 4.0-5.3 crustal earthquakes at low depth (8-9 km) that occurred in Qinghai-Tibet Plateau. Firstly, PWV retrieval using Global Navigation Satellite System (GNSS) technology is performed, showing that its root mean square error (RMSE) is less than 1.8 mm against radiosonde (RS) data or European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis 5 (ERA5) PWV data. The PWV change derived from the nearest GNSS stations around the hypocenter during the earthquakes shows anomalies, and the results reveal that PWV anomalies occurred after the earthquakes, mainly obeying a trend of increasing first and then decreasing. In addition, LST increases three days before PWV peak with a thermal anomaly of 12 °C higher than that of previous days. Robust Satellite Technique (RST) algorithm and ALICE index on Moderate Resolution Imaging Spectroradiometer (MODIS) LST products are introduced to analyze the correlation between the abnormality of LST and PWV. Based on ten-year background field data (2012-2021), the results show that LST during the earthquake has more thermal anomaly occurrences than in previous years. The more severe the LST thermal anomaly is, the higher the probability of a PWV peak occurring.

A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation.

The use of cell and tissue-based methods in basic, applied and regulatory science has been increasing exponentially. Animal-derived components, including serum, coating materials, growth factors and antibodies are routinely used in cell/tissue cultures and in general laboratory practices. In addition to ethical issues, the use and production of animal-derived materials and reagents raises many scientific concerns, generally associated with presence of undefined components and batch-to-batch variability, which may compromise experimental reproducibility. On the other hand, non-animal materials and reagents, such as human cells, alternatives to animal sera or non-animal recombinant antibodies, are becoming increasingly available, and their use is encouraged by the EU Directive 2010/63 and the Guidance Document on Good In vitro Method Practices (GIVIMP), published by the Organization for Economic Cooperation and Development (OECD). In an effort to map the current state of use of animal-derived reagents across different sectors and to identify the obstacles possibly hampering the implementation of non-animal derived alternatives, a global online survey addressed to scientists working on in vivo, in vitro, in silico methods, in academia as well as pharmaceutical or cosmetic companies, was conducted with the goal to understand: 1) the most commonly used animal-derived materials and reagents, 2) the main issues associated with the production and use of animal-derived materials and reagents, 3) the current level of knowledge on available non-animal alternative materials and reagents, and 4) what educational and information sources could be most useful or impactful to disseminate knowledge on non-animal alternatives. This paper provides an overview of the survey replies and discusses possible proposals to increase awareness, acceptance and use of non-animal ingredients.

Relative Positioning in Remote Areas Using a GNSS Dual Frequency Smartphone.

The use of GPS positioning and navigation capabilities in mobile phones is present in our daily lives for more than a decade, but never with the centimeter level of precision that can actually be reached with several of the most recent smartphones. The introduction of the new GNSS systems (Global Navigation Satellite Systems), the European system Galileo, is opening new horizons in a wide range of areas that rely on precise georeferencing, namely the mass market smartphones apps. The constant growth of this market has brought new devices with innovative capabilities in hardware and software. The introduction of the Android 7 by Google, allowing access to the GNSS raw code and phase measurements, and the arrival of the new chip from Broadcom BCM47755 providing dual frequency in some smartphones came to revolutionize the positioning performance of these devices as never seen before. The Xiaomi Mi8 was the first smartphone to combine those features, and it is the device used in this work. It is well known that it is possible to obtain centimeter accuracy with this kind of device in relative static positioning mode with distances to a reference station up to a few tens of kilometers, which we also confirm in this paper. However, the main purpose of this work is to show that we can also get good positioning accuracy using long baselines. We used the ability of the Xiaomi Mi8 to get dual frequency code and phase raw measurements from the Galileo and GPS systems, to do relative static positioning in post-processing mode using wide baselines, of more than 100 km, to perform precise surveys. The results obtained were quite interesting with RMSE below 30 cm, showing that this type of smartphone can be easily used as a low-cost device, for georeferencing and mapping applications. This can be quite useful in remote areas where the CORS networks are not dense or even not available.

Tentativas de suicídio atendidas em um hospital de trauma em Curitiba-PR / Suicide attempts treated at a trauma hospital in Curitiba-PR

RESUMO Introdução: O objetivo do nosso estudo foi investigar os fatores epidemiológicos envolvidos nas tentativas de suicídio atendidas em um hospital de trauma em Curitiba-PR entre janeiro de 2014 a dezembro de 2017. Métodos: Trata-se de um estudo retrospectivo realizado com base na avaliação de fichas de notificação obrigatória e banco de dados do hospital. Foram incluídos pacientes atendidos por tentativas de suicídio, maiores de 18 anos atendidos num período de 48 meses. Foram analisados dados como sexo, idade, estado civil, meio de agressão, natureza da lesão, reincidência da tentativa e desfecho. 283 pacientes foram incluídos no estudo e analisados comparativamente ao perfil característico descrito na literatura. Resultados: Observou-se no período estudado um constante crescimento no número das tentativas, significativamente maior no sexo feminino. Apesar desse aumento em mulheres, houve predomínio de pacientes do sexo masculino 1,3:1 (p < 0,005). A idade média foi de 34,6 ± 13,25 anos e não apresentou diferença significativa entre os sexos. De modo geral, o principal meio utilizado foi objeto perfurocortante (32,8%), seguido por intoxicação (31,8%), homens utilizaram mais objetos cortantes e mulheres envenenamento. Do total de pacientes, 9 (3,2%) foram a óbito obtendo-se uma relação suicídios-tentativas de suicídio de 1:31. Conclusão: O perfil do paciente que tenta suicídio mudou em comparação a estudos anteriores. Embora o número de homens permaneça superior, o número de mulheres está crescendo. O aumento nos índices sugere que há necessidade de melhora das políticas públicas. PALAVRAS-CHAVE: Tentativa de suicídio, serviços médicos de emergência, centros de traumatologia

ABSTRACT Introduction: The aim of our study was to investigate the epidemiological factors involved in suicide attempts seen at a trauma hospital in Curitiba-PR between January 2014 and December 2017. Methods: This is a retrospective study based on the evaluation of hospital mandatory notification forms and database. Patients over 18 years of age who were seen for suicide attempts along a 48-month period were included. Data such as sex, age, marital status, means of aggression, nature of injury, recurrence of attempt, and outcome were analyzed. A total of 283 patients were included in the study and analyzed against the characteristic profile described in the literature. Results: During the study period, there was a constant increase in the number of attempts, significantly higher in females. Despite this increase in women, there was a predominance (1.3:1) of male patients (p < 0.005). The mean age was 34.6 ± 13.25 years and there was no significant gender difference. In general, the main means used was sharp objects (32.8%), followed by intoxication (31.8%), men used more sharp objects, while women, poisoning. Of the total number of patients, 9 (3.2%) died, with a suicides-attempts ratio of 1:31. Conclusion: The profile of the suicide attempt patient has changed compared to previous studies. While the number of men remains higher, the number of women is growing. The increase in indices suggests that public policies should be improved. KEYWORDS: Suicide attempt, emergency medical services, trauma centers

APP Binds to the EGFR Ligands HB-EGF and EGF, Acting Synergistically with EGF to Promote ERK Signaling and Neuritogenesis.

The amyloid precursor protein (APP) is a transmembrane glycoprotein central to Alzheimer's disease (AD) with functions in brain development and plasticity, including in neurogenesis and neurite outgrowth. Epidermal growth factor (EGF) and heparin-binding EGF-like growth factor (HB-EGF) are well-described neurotrophic and neuromodulator EGFR ligands, both implicated in neurological disorders, including AD. Pro-HB-EGF arose as a putative novel APP interactor in a human brain cDNA library yeast two-hybrid screen. Based on their structural and functional similarities, we first aimed to verify if APP could bind to (HB-)EGF proforms. Here, we show that APP interacts with these two EGFR ligands, and further characterized the effects of APP-EGF interaction in ERK activation and neuritogenesis. Yeast co-transformation and co-immunoprecipitation assays confirmed APP interaction with HB-EGF. Co-immunoprecipitation also revealed that APP binds to cellular pro-EGF. Overexpression of HB-EGF in HeLa cells, or exposure of SH-SY5Y cells to EGF, both resulted in increased APP protein levels. EGF and APP were observed to synergistically activate the ERK pathway, crucial for neuronal differentiation. Immunofluorescence analysis of cellular neuritogenesis in APP overexpression and EGF exposure conditions confirmed a synergistic effect in promoting the number and the mean length of neurite-like processes. Synergistic ERK activation and neuritogenic effects were completely blocked by the EGFR inhibitor PD 168393, implying APP/EGF-induced activation of EGFR as part of the mechanism. This work shows novel APP protein interactors and provides a major insight into the APP/EGF-driven mechanisms underlying neurite outgrowth and neuronal differentiation, with potential relevance for AD and for adult neuroregeneration.

A Picture of Breast Reconstruction in a Public Oncology Hospital in Latin America: A Ten-Year Experience.

OBJECTIVE: Breast cancer is the most frequent malignant tumor among women worldwide, with the sole exception of non-melanoma skin cancer. Currently, one of the most common treatments in Brazil is modified radical mastectomy, which, although effective, leads to both physical and psychological complications. In this context, breast reconstruction seeks to restore the functional and psychosocial health of women. This study aims to investigate the characteristics of breast reconstructions after mastectomy by comparing immediate and delayed reconstructions. MATERIALS AND METHODS: This is a retrospective observational study, which was performed by analyzing the electronic medical records of the Erasto Gaertner Hospital in Curitiba, Brazil, from between January 2007 and December 2017. RESULTS: After applying exclusion criteria, we analyzed a total of 268 medical records from January 2010 to December 2017. The most frequent histological type was invasive ductal carcinoma. Patients treated after 2014 had a higher number of immediate reconstructions, and the most commonly used method was alloplastic reconstruction using expanders (66.5%). There was no significant difference in the frequency of immediate or late complications between patients who opted for immediate or delayed reconstructions. The most common immediate complication was surgical wound dehiscence, and the use of neoadjuvant chemotherapy was not associated with a higher rate of complications in immediate reconstructions. CONCLUSION: The current preference is for immediate reconstructions with breast tissue expanders in combination with chemotherapy, which follows a trend in Brazil and worldwide that has been identified in the literature. Finally, the growth in immediate reconstructions with no associated increase in complications demonstrates the effectiveness of this practice.

The Relevance of In Silico, In Vitro and Non-human Primate Based Approaches to Clinical Research on Major Depressive Disorder.

Major depressive disorder (MDD) is the most severe form of depression and the leading cause of disability worldwide. When considering research approaches aimed at understanding MDD, it is important that their effectiveness is evaluated. Here, we assessed the effectiveness of original studies on MDD by rating their contributions to subsequent medical papers on the subject, and we compared the respective contribution of findings from non-human primate (NHP) studies and from human-based in vitro or in silico research approaches. For each publication, we conducted a quantitative citation analysis and a systematic qualitative analysis of the citations. In the majority of cases, human-based research approaches (both in silico and in vitro) received more citations in subsequent human research papers than did NHP studies. In addition, the human-based approaches were considered to be more relevant to the hypotheses and/or to the methods featured in the citing papers. The results of this study suggest that studies based on in silico and in vitro approaches are taken into account by medical researchers more often than are NHP-based approaches. In addition, these human-based approaches are usually cheaper and less ethically contentious than NHP studies. Therefore, we suggest that the traditional animal-based approach for testing medical hypotheses should be revised, and more opportunities created for further developing human-relevant innovative techniques.

Development of physical modelling tools in support of risk scenarios: A new framework focused on deep-sea mining.

Deep-sea mining has gained international interest to provide materials for the worldwide industry. European oceans and, particularly, the Portuguese Exclusive Economic Zone present a recognized number of areas with polymetallic sulphides rich in metals used in high technology developments. A large part of these resources are in the vicinity of sensitive ecosystems, where the mineral extraction can potentially damage deep-ocean life services. In this context, technological research must be intensified, towards the implementation of environmental friendly solutions that mitigate the associated impacts. To reproduce deep-sea dynamics and evaluate the effects of the mining activities, reliable numerical modelling tools should be developed. The present work highlights the usefulness of a new framework for risk and impact assessment based on oceanographic numerical models to support the adoption of good management practices for deep-sea sustainable exploitation. This tool integrates the oceanic circulation model ROMS-Agrif with the semi-Lagrangian model ICHTHYOP, allowing the representation of deep-sea dynamics and particles trajectories considering the sediments physical properties. Numerical simulations for the North Mid-Atlantic Ridge region, revealed the ability of ROMS-Agrif to simulate real deep-sea dynamics through validation with in situ data. Results showed a strong diversity in the particle residence time, with a dependency on their density and size but also on local ocean conditions and bottom topography. The highest distances are obtained for the smaller and less dense particles, although they tend to be confined by bathymetric constrains and deposited in deepest regions. This work highlights the potential of this modelling tool to forecast laden plume trajectories, allowing the definition of risk assessment scenarios for deep-sea mining activities and the implementation of sustainable exploitation plans. Furthermore, the coupling of this numerical solution with models of biota inhabiting deep-sea vent fields into ecosystem models is discussed and outlined as cost-effective tools for the management of these remote ecosystems.

Sea level anomaly in the North Atlantic and seas around Europe: Long-term variability and response to North Atlantic teleconnection patterns.

Sea level anomaly (SLA), provided globally by satellite altimetry, is considered a valuable proxy for detecting long-term changes of the global ocean, as well as short-term and annual variations. In this manuscript, monthly sea level anomaly grids for the period 1993-2013 are used to characterise the North Atlantic Ocean variability at inter-annual timescales and its response to the North Atlantic main patterns of atmospheric circulation variability (North Atlantic Oscillation, Eastern Atlantic, Eastern Atlantic/Western Russia, Scandinavian and Polar/Eurasia) and main driven factors as sea level pressure, sea surface temperature and wind fields. SLA variability and long-term trends are analysed for the North Atlantic Ocean and several sub-regions (North, Baltic and Mediterranean and Black seas, Bay of Biscay extended to the west coast of the Iberian Peninsula, and the northern North Atlantic Ocean), depicting the SLA fluctuations at basin and sub-basin scales, aiming at representing the regions of maximum sea level variability. A significant correlation between SLA and the different phases of the teleconnection patterns due to the generated winds, sea level pressure and sea surface temperature anomalies, with a strong variability on temporal and spatial scales, has been identified. Long-term analysis reveals the existence of non-stationary inter-annual SLA fluctuations in terms of the temporal scale. Spectral density analysis has shown the existence of long-period signals in the SLA inter-annual component, with periods of ~10, 5, 4 and 2years, depending on the analysed sub-region. Also, a non-uniform increase in sea level since 1993 is identified for all sub-regions, with trend values between 2.05mm/year, for the Bay of Biscay region, and 3.98mm/year for the Baltic Sea (no GIA correction considered). The obtained results demonstrated a strong link between the atmospheric patterns and SLA, as well as strong long-period fluctuations of this variable in spatial and temporal scales.

Contribution of animal models to contemporary understanding of Attention Deficit Hyperactivity Disorder.

Attention Deficit Hyperactivity Disorder (ADHD) is a poorly understood neurodevelopmental disorder of multifactorial origin. Animal-based research has been used to investigate ADHD aetiology, pathogenesis and treatment, but the efficacy of this research for patients has not yet been systematically evaluated. However, such evaluation is important, given the resource consumption and ethical concerns incurred by animal use. Accordingly, we used the citation tracking facility within Web of Science to locate original research performed on animal models related to ADHD, prior to 2010. Human medical papers citing those animal studies were carefully analyzed by two independent raters to evaluate the contribution of the animal to the human studies. 211 publications describing relevant animal studies were located. Approximately half (3,342) of their 6,406 citations were by other animal studies. 446 human medical papers cited 121 of these 211 animal studies, a total of 500 times. 254 of these 446 papers were human studies of ADHD. However, only eight animal papers (cited 10 times) were relevant to the hypothesis of the human medical study in question. Three of these eight papers described results from both human and animal studies, but their citations solely referred to the human data. Five animal research papers were relevant to the hypotheses of the applicable human medical papers. Citation analysis indicates that animal research has contributed very little to contemporary understanding of ADHD. To ensure optimal allocation of Research & Development funds targeting this disease the contribution of other research methods should be similarly evaluated.

Accuracy assessment of the integration of GNSS and a MEMS IMU in a terrestrial platform.

MEMS Inertial Measurement Units are available at low cost and can replace expensive units in mobile mapping platforms which need direct georeferencing. This is done through the integration with GNSS measurements in order to achieve a continuous positioning solution and to obtain orientation angles. This paper presents the results of the assessment of the accuracy of a system that integrates GNSS and a MEMS IMU in a terrestrial platform. We describe the methodology used and the tests realized where the accuracy of the positions and orientation parameters were assessed using an independent photogrammetric technique employing cameras that integrate the mobile mapping system developed by the authors. Results for the accuracy of attitude angles and coordinates show that accuracies better than a decimeter in positions, and under a degree in angles, can be achieved even considering that the terrestrial platform is operating in less than favorable environments.

A model for educational simulation of the effect of oxytocin on uterine contractions.

Fetal oxygenation is sometimes compromised due to hyperstimulation of uterine contractions (UC) following labor augmentation with oxytocin. We present a model for educational simulation that incorporates the pharmacokinetic-pharmacodynamic properties of oxytocin, reproducing the effect of this drug on UC features. Six UC tracings were generated, reflecting different relevant situations. Three independent experts identified correctly the simulated situations in all tracings and attributed an average realism score of 9.4 (0-10). The model presented for simulation of the effect of oxytocin on UC provides sufficiently realistic results to be used in healthcare education and can easily be adapted to different patients and educational scenarios.

Sensor integration in a low cost land mobile mapping system.

Mobile mapping is a multidisciplinary technique which requires several dedicated equipment, calibration procedures that must be as rigorous as possible, time synchronization of all acquired data and software for data processing and extraction of additional information. To decrease the cost and complexity of Mobile Mapping Systems (MMS), the use of less expensive sensors and the simplification of procedures for calibration and data acquisition are mandatory features. This article refers to the use of MMS technology, focusing on the main aspects that need to be addressed to guarantee proper data acquisition and describing the way those aspects were handled in a terrestrial MMS developed at the University of Porto. In this case the main aim was to implement a low cost system while maintaining good quality standards of the acquired georeferenced information. The results discussed here show that this goal has been achieved.

A model for educational simulation of the evolution of uterine contractions during labor.

Electronic fetal monitoring remains an important tool in labor ward settings, providing continuous information on fetal heart rate and maternal uterine contractions. A prompt detection of abnormalities in these signals is essential for the timely resolution of situations that may put both mother and fetus at risk. Uterine contraction signals provide information that is important to evaluate the onset and progress of labor, as well as the significance of certain fetal heart rate abnormalities. We present a model for educational simulation of the spontaneous evolution of uterine contractions during labor, which combines a previously published signal generator with literature-based pre-programmed scripts for educationally relevant scenarios. This model is an essential component of a high-fidelity simulator of intrapartum emergencies, aimed to improve the competency of healthcare providers. Real and simulated tracings were presented to three independent clinical experts who judged simulated signals to be indistinguishable or negligibly different from real tracings.

An intrauterine pressure generator for educational simulation of labour and delivery.

Simulation provides a risk free and controllable environment for training of healthcare providers. The limited realism of available simulators and training programs impedes immersive training in obstetric emergencies. In developed countries, intrapartum monitoring in high-risk cases involves continuous evaluation of foetal heart rate and uterine contractions signals. We present an essential component of a high-fidelity simulator for normal and critical situations in labour and delivery, namely an intrauterine pressure generator. The signal model behind the generator consists of a truncated Gaussian curve with the programmable features: amplitude, frequency, duration, and resting tone. Through analysis of 44h of physiological data, we demonstrate that the natural variability of these features and of the baseline pressure can be approximated by deterministic trends and stationary stochastic processes. Signal parameters can be controlled by simulation instructors, scripts, or other models to reflect different patients, pathologies, and evolving clinical situations. Twelve 40-min tracings reflecting three different patients in labour were presented to three clinical experts, who attributed similar realism scores to simulated and to real tracings.